BRD3 (BD1), GST-tag Recombinant

Catalog #

31032

$305 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human Bromodomain containing 3, also known as BRD3 or RING3L, GenBank Accession No. NM_007371, a.a. 29 – 145 corresponding to bromodomain 1 with N-terminal GST-tag, MW = 40.7 kDa, expressed in an E. coli expression system.

This product has been cited 3 times.

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Synonyms

Bromodomain containing 3, BRD3, RING3L, BRD-3

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

BRD3 (BD1) Binding to the Acetylated Ligand

Product Info

Storage and Usage

Citations3

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

40.7 kDa

Amino Acids

29-145

Genbank #

NM_007371

UniProt #

Q15059

Tag(s)

N-terminal GST-tag

References

1. Gamsjaeger, R., et al., Mol Cell Biol. 2011 Jul; 31(13): 2632-2640.
2. Lamonica, JM, et al., Proc Natl Acad Sci USA. 2011 May 31; 108(22): E159-168.

Storage/Stability

>6 months at –80°C.

Assay Conditions

BRD3 protein is incubated with biotinylated acetylhistone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA^® (R) GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen^®(R) Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only

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Datasheets/Protocols

31032_Lot120216.pdf 31032_Lot130416.pdf

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BRD3 (BD1), GST-tag Recombinant

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