BRD3 (BD1+BD2), His-tag Recombinant

Catalog #

31034

$305 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human Bromodomain containing 3, also known as BRD3 or RING3L, GenBank Accession No. NM_007371, a.a. 29 – 417 corresponding to bromodomains 1 and 2, with N-terminal His-tag, MW = 44.2 kDa, expressed in an E. coli expression system

This product has been cited 1 time.

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Synonyms

Bromodomain containing 3, BRD3, RING3L, BRD-3

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

BRD3-BD1,BD2

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

E. coli

Purity

≥37%

Format

Aqueous buffer solution

Formulation

40 mM HEPES, pH 7.2, 290 mM NaCl, and 20% glycerol.

44.2 kDa

Amino Acids

29-471

Genbank #

NM_007371

UniProt #

Q15059

Tag(s)

N-terminal His-tag

References

Gamsjaeger, R., et al., Mol Cell Biol. 2011 Jul; 31(13): 2632-2640.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

BRD3 protein is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA^® Ni acceptor beads (PerkinElmer) are added, followed by Alphascreen^®Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only

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Datasheets/Protocols

31034_Lot140207-1.pdf 31034_Lot120807-SC.pdf

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BRD3 (BD1+BD2), His-tag Recombinant

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