BPTF/FALZ TR-FRET Assay Kit

Catalog #

32632

$1,025 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The BPTF/FALZ TR-FRET Assay Kit is designed to measure the inhibition of the binding of BPTF, also known as FALZ, to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BPTF, substrate, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against BPTF/FALZ? Check out our Bromodomain Screening Services.

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Synonyms

BPTF, FALZ

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Product Data Gallery

Interaction of BPTF (BPS Bioscience #31134) with BET Ligand and inhibition by bromosporine (BPS Bioscience #27612).

Product Info

Storage and Usage

Citations

Assay Kit Format

TR-FRET

Supplied As

Kit comes in a convenient format with purified BPTF/FALZ, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions

Materials Required But Not Supplied

Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

Format

Catalog #	Component	Amount	Storage
31134	BPTF(FALZ), GST-tag	10 µg	-80°C	Avoid freeze/ thaw cycles!
33000	BET Bromodomain Ligand	50 µl	-80°C
33005	Non-acetylated Ligand 1	15 µl	-80°C
	Tb-labeled donor	2x10 µl	-20°C
	Dye-labeled acceptor	2x10 µl	-20°C
33012	3x BRD TR-FRET Assay Buffer 1	4 ml	-20°C
79969	White Nonbinding low volume microtiter plate	1	Room Temp.

UniProt #

Q12830

References

Filippakopoulos, P., et al., Cell 2012; 149:214.

Storage/Stability

At least 6 months from date of receipt when stored as directed. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit datasheet for detailed protocol.

Applications

Useful for screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Notes

The final concentration of DMSO should not exceed 0.5%.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only