ADAM17 Fluorogenic Assay Kit

Catalog #

78000

$465 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The key to the ADAM17 Fluorescent Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for ADAM17 reactions. A sample containing ADAM17 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex = 485 nm, λem = 530 nm) is measured using a plate reader.

Need us to run inhibitor screens or profile your compounds against ADAM17? Check out our Protease Screening Services.

This product has been cited 1 time.

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Synonyms

Disintegrin And Metalloproteinase Domain-Containing Protein, Tumor Necrosis Factor, Alpha, Converting Enzyme, Snake Venom-Like Protease, TNF-Alpha Convertase, ADAM-17

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Product Data Gallery

Product Info

Storage and Usage

Citations1

Assay Kit Format

Fluorogenic

Supplied As

The ADAM17 Fluorogenic Assay Kit is provided in a convenient 96-well format, with purified ADAM17, ADAM Fluorogenic Substrate, and ADAM assay buffer for 96 enzyme reactions.

Materials Required But Not Supplied

Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog #	Component	Amount	Storage
	ADAM17	3 µg	-80°C	Avoid freeze/ thaw cycles!
	ADAM Fluorogenic Substrate (1 mM)	50 µl	-80°C
78001	1X ADAM Assay Buffer	5 ml	-20°C
79685	96-well black plate	1	Room Temp

UniProt #

P78536

Background

ADAM17 (ADAM Metallopeptidase Domain 17) is part of the ADAM family of disintegrins and metalloproteases. Initially identified as TNF-α converting enzyme (TACE), ADAM 17 has been linked to a number of diverse signaling pathways. It cleaves ectodomains of various transmembrane proteins and regulates cytokine shedding. It also plays a role in inflammatory skin and bowel disease.

References

1. Scheller, Jürgen, et al. 2011. "ADAM17: a molecular switch to control inflammation and tissue regeneration." Trends in Immunology 32(8): 380-387.
2. Blaydon, Diana C., et al. 2011. "Inflammatory skin and bowel disease linked to ADAM17 deletion." New England Journal of Medicine 365(16): 1502-1508.

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See assay protocol for detailed instrcutions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Protease

Regulatory Status

Research Use Only