ADAM10 Fluorogenic Assay Kit
The key to the ADAM10 Fluorogenic Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for ADAM10 reactions. A sample containing ADAM10 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=485±10 nm, λem=530±10 nm) is measured using a plate reader.
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- Luminometer or fluorescent microplate reader capable of reading chemiluminescence
- Adjustable micropipettor and sterile tips
- Rotating or rocker platform
| Catalog # | Component | Amount | Storage | |
| ADAM10* | 3 µg | -80°C | Avoid freeze/ thaw cycles! |
|
| ADAM Fluorogenic Substrate (1 mM) | 50 µl | -80°C | ||
| 78001 | 1X ADAM Assay Buffer | 5 ml | -20°C | |
| 79685 | 96-well black plate | 1 | Room Temp |
|
*The concentration of ADAM10 is lot-specific and will be indicated on the tube containing the enzyme.
ADAM10 or ADAM Metallopeptidase Domain 10, is an enzyme that is involved in a diverse array of signaling pathways. It is part of the ADAM family of disintegrins and metalloproteases. It also plays a role in inflammatory disease and cancer.
1. Scheller, J., et al. 2011. "ADAM10: a molecular switch to control inflammation and tissue regeneration." Trends in Immunology 32(8): 380-387.
2. Blaydon, D.C., et al. 2011. "Inflammatory skin and bowel disease linked to ADAM10 deletion." New England Journal of Medicine 365(16): 1502-1508.
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