Wee1 Kinase Assay Kit

Catalog #

79909

$560 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Wee1 Kinase Assay Kit is designed to measure Wee1 kinase activity for screening and profiling applications using Kinase-Glo® Kinase Assay as a detection reagent. The Wee1 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant Wee1 enzyme (amino acids 215-646), Wee1 Substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Need us to run inhibitor screens or profile your compounds against Wee1? Check out our Kinase Screening Services or DNA Replication and Repair Screening Services.

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Synonyms

Wee1A Kinase, Wee1 Protein, Wee1-like protein kinase, Wee1A kinase

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Product Data Gallery

Inhibition of Wee1 by the inhibitor MK-1775.

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

Kinase-Glo® Kinase Assay (Promega #V6071)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
40412	Wee1, GST-Th-Tag*	15 μg	-80°C
79334	5x Kinase Assay Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	100 µl	-20°C
101574	Wee1 Substrate (5 mg/ml)	200 µl	-80°C
79696	White 96-well plate	1	Room Temp.

*The concentration of the protein is lot-specific and will be indicated on the tube

UniProt #

P30291

Background

Wee1 (also known as WEE1 G2 checkpoint kinase) belongs to the serine-threonine kinase family of proteins. Wee1 plays a crucial role in regulating G2/M transition through phosphorylation of cyclin dependent kinase (CDKs), allowing DNA damage to be repaired. that is overexpressed in many types of cancer such as luminal and HER2-positive breast cancer, hepatocellular carcinomas, and glioblastomas. Classical cancer therapies, such as chemotherapy and radiotherapy, induce DNA damage, and cells trigger the checkpoint in cell cycle, such as Wee1. This can lead to cancer cell resistance. Inhibiting Wee1 prevents cells from repairing DNA damage due to unchecked replication, suggesting that combination therapeutics with DNA damaging reagents and Wee1 inhibitors may be effective against cancer.

References

Parker L., et al., 1992 Science, 257(5078): 1955.
Lundgreen K., et al., 1991 Cell, 64(6): 1111-1122.
Vakili-Samiani S., et al., 2022 Mutat Res 824: 111776.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only