UBE1 Inhibitor Screening Assay Kit
The UBE1 Inhibitor Screening Assay Kit is a 96-well format assay designed to measure the activity of the ubiquitin activating enzyme UBE1 for screening and profiling applications, using the Kinase-Glo® MAX as detection reagent. The kit contains enough purified UBE1 protein, Ubiquitin, ATP, and kinase assay buffer for 100 reactions
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- Kinase-Glo® MAX (Promega #V6071)
- Dithiothreitol (DTT, 0.5 M; optional)
- Adjustable micropipettor and sterile tips
- Luminescence Plate Reader
- 30°C incubator
| Catalog # | Reagent | Amount | Storage |
| 80301 | UBE1, FLAG-tag* | 150 µg | -80°C |
| 79334 | 5x Kinase assay buffer | 1.5 ml | -20°C |
| 79686 | ATP (500 μM) | 100 µl | -20°C |
| Ubiquitin (500 µM) | 100 µl | -20°C | |
| 79696 | 96-well white microplate | 1 | Room Temp |
*The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
UBE1 (also known as Ubiquitin Activating enzyme 1, UBA1 or Ubiquitin like modifier Activating enzyme 1) belongs to the family of ubiquitin activating enzymes, responsible for the first reaction in the multi-step protein ubiquitination process. UBE1 forms a thioester bond with ubiquitin in an ATP dependent reaction. In humans only two ubiquitin activating enzymes are known, UBE1 and UBA6 and through their role in the ubiquitination pathway, they are involved in the regulation of several critical cellular processes, such as cell cycle and signal transduction. Mutations in UBE1 have been identified in spinal muscular atrophy and cancer. UBE1 inhibitors can prove crucial for the treatment of neurodegenerative diseases and cancer.
1. Ramser, J., et al. 2008. "Rare missense and synonymous variants in UBE1 are associated with X-linked infantile spinal muscular atrophy." Amer. J.Human Genetics 82(1): 188-193.
2. Leidecker, O., et al. 2012. "The ubiquitin E1 enzyme Ube1 mediates NEDD8 activation under diverse stress conditions." Cell Cycle 11(6): 1142-1150.
Application Reference(s):
1. Data Sheet UBE1, FLAG-Tag (2020)
2. UBE1 Inhibitor Screening Assay Kit (2020)
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