TSSK2, GST-tag Recombinant

Catalog #
40290
$410 *
Size: 10 µg
Qty
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Description

Recombinant human TSSK2, full length, with an N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system. The gene accession number is NM_053006.

Synonyms
DGS-G, SPOGA2, STK22B, FLJ38613, testis-specific serine kinase 2
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
Sf9 insect cells
Purity
≥90%
Format
Aqueous buffer solution
Formulation
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
MW
66 kDa
Amino Acids
full length
Genbank #
NM_053006
UniProt #
Q96PF2
Tag(s)
N-terminal GST-tag
Background
TSSK2 is an intronless serine-threonine kinase that was originally detected on mouse chromosome 16 (1). TSSK2 and TSSK1 share 72% overall amino acid identity and 83% identity in the kinase domain (2). Real-time PCR showed abundant TSSK2 expression in testis and lower levels in heart, brain, and placenta while Western blot analysis detected TSSK2 at the predicted molecular mass of 40 kDa in human sperm and testis. Co-immunoprecipitation and yeast 2-hybrid analysis show that TSSK2 can interact with a protein TSKS, that also acts as a substrate for this protein kinase.
References
1. Nayak, S. et al: Immunohistochemical analysis of the expression of two serine-threonine kinases in the maturing mouse testis. Mech Dev. 1998 Jun;74(1-2):171-4.
2. Hao, Z. et al: Expression analysis of the human testis-specific serine/threonine kinase (TSSK) homologues: a TSSK member is present in the equatorial segment of human sperm. Molec. Hum. Reprod. 10: 433-444, 2004.