TRKC Kinase Assay Kit
The Tropomyosin receptor kinase C (TRKC) Kinase Assay Kit is designed to measure TRKC kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
- Kinase-Glo MAX (Promega #V6071)
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
| Catalog # | Name | Amount | Storage |
| 40282 | TRKC, GST-tag Recombinant* | 10 µg | -80°C |
| 79334 | Kinase assay buffer 1 (5x) | 1.5 ml | -20°C |
| 79686 | ATP (500 µM) | 100 µl | -20°C |
| 40217 | PTK substrate Poly (Glu:Tyr 4:1) (10 mg/ml) | 100 µl | -20°C |
| 79696 | White 96-well plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube.
Tropomyosin receptor kinase C (TRKC), also known as neurotrophic tyrosine kinase receptor type 3, is a member of the receptor tyrosine kinase family that mediates neurotrophin-activated signaling. TRKC is a functional coordinator in the activation of a host of signaling cascades including the mitogen-activated protein kinase (MAPK) pathway that leads to cell differentiation. Most recently, it has been shown that TRKC levels in hippocampal neurons dictate TRKC-mediated synapse density. TRKC expression levels are known to play a role in tumorigenesis as well as neurodegenerative diseases. Studies involving TRKC may lead to further understanding of disease pathology and to therapeutic treatments.
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