TRβ-GAL4 Luciferase Reporter HEK293 Cell Line (Thyroid Hormone Receptor β Pathway)

Catalog #

82175

$10,500 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The TRβ-GAL4 Luciferase Reporter HEK293 Cell Line is a HEK293 cell line expressing firefly luciferase under the control of the GAL4 upstream activation sequence (UAS) with constitutive expression of human thyroid receptor β ligand binding domain (TRβ LBD, amino acids 173-461) fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD). This system allows specific detection of thyroid hormone-induced activation of the thyroid receptor β with low cross-reactivity from other nuclear receptors. This cell line has been validated by stimulation with triiodothyronine (T-3).

Interested in screening and profiling inhibitors, blocking antibodies, or activators of thyroid hormone receptor β without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

thyroid hormone receptor beta, TRb, THRb, PRTH, THRI

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Product Data Gallery

Dose response curve of TRβ-GAL4 Luciferase Reporter HEK293 Cell Line to T-3 (96-well format).

Dose response curve of TRβ-GAL4 Luciferase Reporter HEK293 Cell Line to T-3 (384-well format).

Activation of TRβ-GAL4 Luciferase Reporter HEK293 Cell Line by MGL-3196 (Resmetirom).

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293, Human Embryonic Kidney, epithelial-like cells, adherent.

Supplied As

Each vial contains ≥1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1M	BPS Bioscience #79723

Materials Required for Cellular Assay

Name	Ordering Information
3, 3’, 5-Triiodo-L-thyronine (T-3)	Cayman #16028
Assay Medium 6B	BPS Bioscience #82202
96-well tissue culture treated white clear-bottom assay plate	Corning #3610
384-well tissue culture treated white clear-bottom assay plate	PerkinElmer #6007680
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Thyroid hormones play an important role in growth, development, and metabolism. These are mediated by two different thyroid hormone receptor (TR) isoforms, TRα and TRβ. Thyroid receptors are transcriptional factors that control various genes by interacting with specific co-activators, co-repressors and DNA sequences. In humans, TRα is the predominant form of the thyroid receptor in the heart, brain, and bone while TRβ is mainly expressed in the liver, kidney and brain. Interestingly, it has been shown that the patients with non-alcoholic steatohepatitis (NASH) display lower TRβ expression in the liver, and more importantly, TRβ agonist treatment decreased liver steatosis and circulating lipids as well as showed metabolic benefits in preclinical models of diabetes and obesity.

References

Paguio A, et al., 2010 Curr Chem Genomics. 4: 43-49.

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Note that using similar but not BPS Bioscience validated reagents can result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, and 1% Penicillin/Streptomycin

Growth Medium 1M (BPS Bioscience #79723): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin, 400 µg/ml of G418, and 0.5 µg/ml of Puromycin.

Media Required for Functional Cellular Assay
Assay Medium: DMEM (without phenol red) medium supplemented with 2% charcoal/dextran treated FBS, 1% Gluta-Max^TM, and 1% Penicillin/Streptomycin

Applications

Screen for TRβ agonists.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Metabolic Enzymes

Regulatory Status

Research Use Only