PTP1B (PTPN1) full length, GST-Tag Recombinant

Catalog #

30009

$325 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Protein tyrosine phosphatase nonreceptor, type 1 (PTP1B, also known as PTPN1), full length encompassing amino acids 1-435 (end). This construct contains an N-terminal GST-tag, linked to the protein via a short peptide corresponding to a thrombin cleavage site. The recombinant protein was affinity purified and tested for activity.

This product has been cited 4 times.

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Synonyms

PTP1B, PTPN1, Protein tyrosine phosphatase 1B, ptp

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PTP1B Activity

Product Info

Storage and Usage

Citations4

Species

Human

Construct

PTP1B (GST-Th-1-435(end))

Host Species/Expression System

E. coli

Purity

≥55%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT

77 kDa

Amino Acids

1-435

Specific Activity

2,000 pmol/min/µg

Genbank #

M31724

UniProt #

P18031

Tag(s)

N-terminal GST-tag

Background

PTP1B, also known as PTPN1, is the founding member of the protein tyrosine phosphatase (PTP) family. PTP1B catalyze the hydrolysis of the phosphate monoesters specifically from tyrosine residues. PTP1B is involved in signaling pathways that regulate a variety of cellular processes including cell growth and oncogenic transformation and has been shown to act as a negative regulator of insulin signaling. PTP1B has also been reported to dephosphorylate epidermal growth factor receptor kinase, which implicates this phosphatase in cell growth control and cell response to interferon stimulation.

References

1. Frangioni et al., Cell, 1992, 68: 545.
2. M. Elchebly et al., Science 1999, 283: 1544.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

20 mM HEPES, pH 7.2, 2 mM EDTA, 1 mM DTT, 25 mM pNPP. The assay was done at room temperature for 1 hour and monitored at Abs415 nM. ε pNPP = 18,000 M-1cm-1 with various amounts of PTP1B.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PTP/Non-rec.

Regulatory Status

Research Use Only