Fluorogenic PTP1B (Catalytic Domain) Assay Kit

Catalog #

79764

$425 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Protein phosphorylation is one of the most important post-translational modification processes. Phosphorylation is reversibly regulated by Protein Kinases (PKs) and Protein Phosphatases (PTPs). PTP1B (PTPN1) is known to catalyze dephosphorylation of insulin receptor kinases and plays a critical role in insulin signaling. The Fluorogenic PTP1B (Catalytic Domain) Assay Kit is designed to inhibitors of the catalytic region of PTP1B in a homogeneous assay with no time-consuming washing steps. Note: To identify inhibitors of the regulatory region of PTP1B, please use our Fluorogenic PTP1B (Full Length) Assay Kit, #79766).

Need us to run inhibitor screens or profile your compounds against PTP1B (Catalytic Domain)? Check out our Protein Tyrosine Phosphatase Screening Services.

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Synonyms

PTPN1, Protein tyrosine phosphatase 1B, ptp

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Fluorogenic

Species

Human

Supplied As

he PTP1B assay kit comes in a convenient 96-well format, with purified PTP1B enzyme, fluorogenic substrate, and PTP assay buffer for 96 enzyme reactions.

Materials Required But Not Supplied

Fluorescent microplate reader capable of reading ex/em=360nm/460nm

Format

Catalog #	Component	Amount	Storage
30010	Recombinant Human PTP1B (1-321)*	≥1 µg	-80°C	*Avoid freeze/ thaw cycles!*
78183	0.5 mM PTP1B Substrate	50 µl	-80°C
79716	5X PTP Assay Buffer	20 ml	-20°C
	0.5 M DTT	200 µl	-20°C
79685	Black, low binding black microtiter plate	1	Room Temperature

*Additional material has been provided for ease of retrieval.

UniProt #

P18031

References

Gee, K.R., et al., Anal Biochem, 1999 Aug 15; 273(1):41-8.

Brown-Shimer, S., et al., 1992 Jan 15; 52(2):478-82.

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Protein Phosphatase

Regulatory Status

Research Use Only