PTP1B (Catalytic Domain) Colorimetric Assay Kit

Catalog #

30019

$425 *

Size: 96 Reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Protein phosphorylation is one of the most important post-translational modification processes. Phosphorylation is reversibly regulated by Protein Kinases (PKs) and Protein Phosphatases (PTPs). PTP1B (PTPN1) is known to catalyze dephosphorylation of insulin receptor kinases and plays a critical role in insulin signaling. The PTP1B (Catalytic Domain) Colorimetric Assay Kit is designed to measure PTP1B activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. The PTP1B assay kit comes in a convenient 96-well format, with purified PTP1B enzyme, colorimetric substrate, and PTP assay buffer for 100 enzyme reactions. The PTP1B (Catalytic Domain) Colorimetric Assay Kit is optimized for both continuous and end-point assay formats.

This product has been cited 6 times.

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Synonyms

PTPN1, Protein tyrosine phosphatase 1B, ptp

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Product Data Gallery

Product Info

Storage and Usage

Citations6

Assay Kit Format

Colorimetric

Supplied As

The PTP1B (Catalytic Domain) Colorimetric Assay Kit comes in a convenient 96-well format, with purified PTP1B enzyme, colorimetric substrate, and PTP assay buffer for 100 enzyme reactions.

Format

Catalog #	Reagent	Amount	Storage
30010	PTP1B (1-321), GST-tag*	10 µg	-80°C	Avoid freeze/thaw cycles!
79716	5x PTP assay buffer	3 ml	-20°C
79693	10x PTP1B Colorimetric Substrate	1 ml	-20°C
79963	Transparent 96-well plate	1	Room Temp

*PTP1B concentration is lot-specific and will be indicated on the tube containing the enzyme.

References

1. Barrett, W.C., et al., Biochem. 1999; 38: 6699-6705.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C (dry ice)

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Protein Phosphatase

Regulatory Status

Research Use Only