PDGFRα (D842V) Assay Kit
Catalog #
79828
$535
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Purchase
Description
The PDGFRα (D842V) Assay Kit is designed to measure PDGFRα (D842V) activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.
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Synonyms
CD140A kit , PDGFR2 kit , MGC74795 kit , Rhe-PDGFRA kit, PDGFRα activity kit
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Product Data Gallery
Product Info
Storage and Usage
Citations
Assay Kit Format
Luminescent
Supplied As
The PDGFRα (D842V) Assay Kit comes in a convenient 96-well format, with enough purified recombinant PDGFRα (D842V), Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 96 enzyme reactions.
Materials Required But Not Supplied
ADP-Glo Kinase Assay (Promega #V6930)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
Format
Catalog Number |
Component |
Amount |
Storage |
|
79633 | PDGFRα (D842V), GST-Tag | 2 µg | -80°C | Avoid freeze/ thaw cycles! |
79334 | 5x Kinase assay buffer | 1.5 ml | -80°C | |
79686 | ATP (500 μM) | 100ml | -20°C | |
40217 | Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml) | 100 µl | -20°C | |
79696 | 96-well plate, white | 1 | Room Temp |
UniProt #
P16234
Background
Platelet-derived growth factor receptor A or PDGFRα has been implicated in regulation of cell growth and survival, apoptosis, and differentiation. It has been identified as a potential target in eosinophilic leukemia cancer, inflammatory breast cancer, and gastrointestinal stromal tumors. The D842V is one of the most predominant of the D842 mutations, which are related to gastrointestinal stromal tumors and are associated with resistance to tyrosine kinase inhibitors.
References
Evans, E., et al. A Precision Therapy Against Cancers Driven by KIT/PDGFRA Mutations; 2017, Science Translational Medicine Nov; 9(414): 1690.