PDGFRα (D842I) Assay Kit

Catalog #

79761

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Platelet-derived growth factor receptor A or PDGFRα has been implicated in regulation of cell growth and survival, apoptosis, and differentiation. It has been identified as a potential target in eosinophilic leukemia cancer, inflammatory breast cancer, and gastrointestinal stromal tumors. The D842 mutation is found in gastrointestinal stromal tumors and is associated with resistance to tyrosine kinase inhibitors. The PDGFRα (D842I) Assay Kit is designed to measure PDGFRα (D842I) activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Need us to run inhibitor screens or profile your compounds against PDGFRα (D842I)? Check out our Kinase Screening Services.

This product has been cited 2 times.

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Synonyms

Platelet Derived Growth Factor Receptor Alpha, CD140 Antigen-Like Family Member A, PDGFR2, CD140a Antigen

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Product Data Gallery

Product Info

Storage and Usage

Citations2

Assay Kit Format

Luminescent

Supplied As

The PDGFRα (D842I) Assay Kit comes in a convenient 96-well format, with enough purified recombinant PDGFRα (D842I), Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 96 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Reagent	Amount	Storage
100202	PDGFRα (D842I), GST-Tag	10 µg	-80°C	*Avoid multiple freeze/ thaw cycles!*
79334	5x Kinase Assay Buffer 1	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
40217	Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	RT

UniProt #

P16234

References

1. Evans, E., et al. A Precision Therapy Against Cancers Driven by KIT/PDGFRA Mutations; 2017, Science Translational Medicine Nov; 9(414): 1690.

2. Joglekar-Javadekar, M., et al. Characterization and Targeting of Platelet-Derived Growth Factor Receptor alpha (PDGFRA) in Inflammatory Breast Cancer (IBC). Neoplasia. 2017 Jul; 19(7):564-573.

3. Corless, C.L., et al. PDGFRA mutations in gastrointestinal stromal tumors: frequency, spectrum and in vitro sensitivity to imatinib. J Clin Oncol. 2005 Aug 10; 23(23):5357-64.

Storage/Stability

Up to 6 months when stored as recommended.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only

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PDGFRα (D842I) Assay Kit

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