PDE1B (Rat), His-GST-tags Recombinant

Catalog #

60009

$455 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Rat PDE1B (GenBank Accession No. NM_022710), full length with N-terminal HIS-GST-tag, MW= 89 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PDE1B, phosphodiesterase 1B, PDE1-B, PDE

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

PDE1B (Rat) inhibition curve using a fluorescence polarization assay (#79662). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Rat

Host Species/Expression System

Sf9 insect cells

Purity

≥35%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 3 mM DTT, 20% glycerol.

89 kDa

Amino Acids

full length

Specific Activity

≥ 6800 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Genbank #

NM_022710

UniProt #

Q01066

Tag(s)

N-terminal His-GST-tags

References

1. Bender A.T. and Beavo J.A. Proc. Natl. Acad. Sci. U.S.A. 103 (2), 460-465 (2006).
2. Bender A.T. et al., Proc. Natl. Acad. Sci. U.S.A. 102 (2), 497-502 (2005).

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE1B at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only