Recombinant HEK293 cell line expressing human PDE1B (phosphodiesterase 1B, accession number NM_000924).
N-terminal His-tagged human PDE1B has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The function of PDE1B was characterized by a cell-based cGMP assay. In this assay system, cells co-express atrial natriuretic peptide (ANP) receptor A (a guanylate cyclase), cyclic nucleotide5gated cation channel CNGA2, and the photoprotein aequorin. Intracellular cGMP level is monitored via aequorin luminescence induced by Ca2+ influx through CNGA2, acting as the intracellular cGMP sensor.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
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Synonyms
PDE1B, phosphodiesterase 1B, PDE1-B, PDE
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Product Data Gallery
PDE1B overexpression reduces cAMP accumulation after forskolin stimulation
Each vial contains ~1 X 106 cells in 1 ml of 10% DMSO.
Format
aqueous solution containing DMSO
Genbank #
NM_000924
UniProt #
Q01064
Mycoplasma Testing
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Background
Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP by
hydrolyzing cAMP and cGMP to their inactive 5' monophosphates. These cyclic
nucleotides play an important role as second messengers in diverse physiological
functions. PDE1B plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE1B hydrolyzes both cAMP and cGMP to nucleoside 5-prime monophosphate. Although it prefers cGMP at low substrate levels, it has a Vmax that is approximately equal for both cGMP and cAMP (Polli, J.W. and Koncaid, R.L., 1994; Yu, J. et al., 1997).
Storage/Stability
Store in liquid nitrogen immediately upon receipt.
Instructions for Use
See data sheet for detailed culturing and assay protocol.
Applications
Useful to monitor cAMP and PDE1B signaling pathway activity and to screen activators or inhibitors of PDE1B.
Shipping Temperature
-80°C
Notes
License Disclosure Visit bpsbioscience.com/license for the label license and other key information about this product.
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