PDE1B, GST-tag Recombinant

Catalog #

60011

$455 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human PDE1B (GenBank Accession No. NM_000924), full length with N-terminal GST-tag, MW= 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 3 times.

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Synonyms

PDE1B, phosphodiesterase 1B, PDE1-B, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE1B inhibition curves using a fluorescence polarization assay (#60311). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations3

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 380 mM NaCl, 1 mM DTT, 10% glycerol

87 kDa

Amino Acids

full length

Specific Activity

≥ 7600 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5?-AMP per minute at 37°C.

Genbank #

NM_000924

UniProt #

Q01064

Tag(s)

N-terminal GST-tag

References

1. Bender A.T. and Beavo J.A. Proc. Natl. Acad. Sci. U.S.A. 103 (2), 460-465 (2006).
2. Bender A.T. et al., Proc. Natl. Acad. Sci. U.S.A. 102 (2), 497-502 (2005).

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE1B at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only