PARP15-FL Chemiluminescent Assay Kit
The PARP15-FL Chemiluminescent Assay Kit is designed to measure the activity of full length PARP15 (poly-(ADP-ribose) polymerase 15) for screening and profiling applications. The PARP15-FL assay kit comes in a convenient 96-well format, with enough recombinant purified full length PARP15 enzyme, reaction substrates, and PARP assay buffer for 100 enzyme reactions.
Figure 1. PARP15-FL Chemiluminescent Assay Kit schematic.
Histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP15 enzyme in an optimized assay buffer. The plate is then treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to PARP15 activity.
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- 1x PBS (Phosphate Buffer Saline) Buffer
- PBST Buffer (1x PBS, containing 0.05% Tween-20)
- Luminometer or microplate reader capable of reading chemiluminescence
- Adjustable micropipettor and sterile tips
- Rotating or rocker platform
Catalog # | Name | Amount | Storage |
100191 | PARP15, GST-Tag* | 2 µg | -80°C |
52029 | 5x Histone Mixture | 1 ml | -80°C |
78371 | PARP Substrate Mixture 2 | 250 µl | -80°C |
80602 | 10x PARP Assay Buffer | 1 ml | -20°C |
79743 | Blocking Buffer 3 | 25 ml | +4°C |
0.5 M DTT | 200 µl | -20°C | |
80611 | Streptavidin-HRP | 100 µl | +4°C |
79670 | ELISA ECL Substrate A (translucent bottle) | 6 ml | Room Temp |
ELISA ECL Substrate B (brown bottle) | 6 ml | Room Temp | |
79837 | 96-well module plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube.
PARP15, also known as poly-(ADP-ribose) polymerase 15 or ARTD7 (diphteria toxin like ADP-ribosyl transferase 7), is part of the PARP family. ADP ribosylation, which is the addition of an ADP-ribose to a protein, is a reversible post-translational modification of proteins mostly involved in the DNA Damage Response (DDR) pathway. Mono-ADP-ribosylation (termed MARylation) is the addition of a unit of ADP-ribose. PARP15 is involved in telomere maintenance, stress response, transcription and immune signaling. Little is known about PARP15, although some inhibitors have been identified. A deeper understanding of the function of this protein in health and disease will benefit the overall oncology field and may bring to light new treatment options.
Zhou X., et al., 2022 Biochemical and Biophysical Research Communications 622: 93-100.