PARG Knockout HeLa Cell Line

Catalog #

82171

$2,500 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

PARG Knockout HeLa Cell Line is a HeLa cell line in which human PARG (Poly ADP-ribose glycohydrolase) long isoforms (PARG₁₁₁, PARG₁₀₂ and PARG₉₉) have been genetically removed using CRISPR/Cas9 genome editing with a lentivirus encoding CRISPR/Cas9 gene and sgRNA (single guide RNA) targeting human PARG.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Poly(ADP-ribose) glycohydrolase, PARG99

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Product Data Gallery

Genomic sequencing of PARG in the PARG Knockout HeLa Cell Line

PARG Expression in PARG Knockout HeLa Cell Line by Western Blot

Product Info

Storage and Usage

Citations

Host Cell Line

HeLa human epithelial cell line. Adherent cells.

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 6	BPS Bioscience #60183

UniProt #

Q86W56

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Poly (ADP-ribose) glycohydrolase (PARG) is a catabolic enzyme involved in the degradation of PARylated chains, releasing ADP-ribose and oligo (ADP-ribose) chains. PAR (poly-ADP ribosylation) homeostasis is regulated by the family of PAR polymerases (PARPs) and PARG in response to cellular stress conditions such as DNA damage response (DDR). PARG activity is linked to cellular responses in inflammation, ischemia, stroke, and cancer. PARG is overexpressed in breast cancer and associated with tumor growth and survival. Decrease in PARG activity can potentiate the effect of current cancer therapies, such as chemotherapy and radiation, making PARG inhibition with selective inhibitors a promising approach in cancer and immunotherapy.

References

Marques M., et al., 2019 Oncogene 38 (12): 2177-2191.
James D. I., et al., 2016 ACS Chem Biol 11 (11): 3179-3190.
Drown B. S., et al., 2018 Cell Chem Bio 25 (12): 1562-1570.

Storage/Stability

Cells will arrive in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Line Culture
Thaw Medium 6 (BPS Bioscience #60183): DMEM medium supplemented with 10% FBS and 1% Penicillin/Streptomycin.

Applications

Use as a negative control when testing PARG inhibitors in HeLa cells.
Study the phenotype of PARG knockout.
Introduce further CRISPR/Cas9-based genetic manipulations in order to understand the interplay between PARG and other partners and pathways.

Shipping Temperature

-80°C

Notes

The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

DNA Replication and Repair

Regulatory Status

Research Use Only