PARG, His-Tag Recombinant

Catalog #

101726

$545 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human PARG (Poly(ADP-ribose) glycohydrolase), full length encompassing amino acids 2-976 (end). The construct contains an N-terminal His-Tag (6xHis). The recombinant protein was affinity purified.

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Synonyms

Poly(ADP-ribose) glycohydrolase, PARG99

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PARG Activity

PARG inhibition curve using a fluorogenic assay (#78858-1). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

PARG (His-2-976(end))

Host Species/Expression System

Sf9

Purity

≥70%

Format

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 610 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 0.5 mM TCEP, and variable imidazole

112 kDa

Amino Acids

2-976(end)

Genbank #

NM_003631.5

UniProt #

Q86W56

Background

PARG is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. It reverses PARP-mediated ADP-ribosylation and plays a role in DNA damage responses. PARG inhibition may prove beneficial in cancer therapy.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Various concentrations of PARG were assayed using Fluorogenic Assay Kit (BPS Bioscience #78858) at room temperature with 4 µM TFMU-ADPr (substrate). The fluorogenic product was measured after 1 hour (Excitation: 385 nm / Emission: 502 nm). Enzyme concentration for IC₅₀: 2.5 ng/rxn (~0.9 nM).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PARP

Regulatory Status

Research Use Only