LysA™ Protease Inhibitor Cocktail Kit
LysA™ Protease Inhibitor Cocktail Kit includes a 100x cocktail of 6 known pan-protease small molecule inhibitors designed to maintain protein integrity during cell lysis and a separate vial of 0.5 M EDTA for optional metalloprotease inhibition. This cocktail contains AEBSF, Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A. AEBSF and Aprotinin are serine proteases inhibitors. Bestatin is an aminopeptidase inhibitor, Pepstatin A inhibits aspartic acid proteases and E-64 acts against cysteine proteases. Leupeptin acts on both serine and cysteine proteases. This product is designed to supplement a cell lysis buffer of choice to prevent post-lysis protein degradation by proteases present in the cell lysate.
Component A: 104 mM AEBSF, 80 μM Aprotinin, 4 mM Bestatin, 1.4 mM E-64, 2 mM Leupeptin, and 1.5 mM Pepstatin A in DMSO.
Component B: 0.5 M EDTA in aqueous solution
Proteases are a large group of enzymes, which catalyze the hydrolytic breakdown of proteins into amino acids or peptides. In living cells proteases are highly regulated and compartmentalized, but upon cell lysis they are released and mixed with other extracted proteins which now became subjected to proteolytic degradation. Inhibition of proteases in cell lysates is important in preserving intact proteins for analysis.
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