PAK2 Recombinant

Catalog #

40073

$515 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human PAK2 (GenBank Accession No. NM_002577), a.a. 227-524, MW=33.5 kDa, expressed in E. coli.

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Product Data Gallery

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥95%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8, 150 mM NaCl, 10% glycerol, 5 mM DTT

33.5 kDa

Amino Acids

227–524

Specific Activity

86 nmoles/min/μg

Genbank #

NM_002577

UniProt #

Q13177

Tag(s)

none

Background

PAK proteins is a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK2 is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. The CDC42 and RAC1 induce autophosphorylation of PAK2, which stimulates sustained phosphorylation of other substrates.

References

1. Coniglio,S.J., et al., Mol. Cell. Biol. 28 (12), 4162-4172 (2008).
2. Koh,W., et al., J. Cell. Sci. 121 (PT 7), 989- 1001 (2008).

Storage/Stability

>6 months at –80°C.

Assay Conditions

Analysis of enzymatic activity was performed according to Zlyte assay protocol (Invitrogen). PAK2 was incubated in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (SER/THR 20, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction for 1 hour at RT. Fluorescence was detected using λexc=460±40 nm and λem=528±20 nm

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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PAK2 Recombinant

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