Chemi-Verse™ PAK2 Kinase Assay Kit

Catalog #

82221

$575 *

Size: 96 reactions

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The Chemi-Verse™ PAK2 Kinase Assay Kit is designed to measure PAK2 (P21 protein (Cdc42/Rac)-activated kinase 2) serine/threonine kinase activity for screening and profiling applications using ADP-Glo^™as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant PAK2 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

●

Synonyms

Gamma-PAK, PAK65, S6/H4 kinase, p21-activated kinase 2 (PAK-2), p58, Serine/threonine-protein kinase PAK 2

●

Product Data Gallery

Inhibition of PAK2 kinase activity by Staurosporine

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
	PAK2*	5 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
	PAKtide, 1 mg/ml	500 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

Q13177

Background

PAK2, also known as P21 protein (Cdc42/Rac)-activated kinase 2, belongs to the group I of the PAK family of serine/threonine kinases. PAK2 can be found both in the cytosol and nucleus of most cells. It can be activated by cellular context dependent partners, and plays a role in several crucial cellular processes, such as cell proliferation and growth. PAK2, in particular, is linked to responses to caspase-mediated apoptosis. The molecule is cleaved in response to cell stress in a caspase-dependent manner, but its non-cleaved form seems to have anti-apoptotic effects. Amongst the PAK proteins, it is the most common protein mutated in cancer, particularly common in lung cancer. Once activated it can modulate the activity of proteins like c-Jun to stimulate melanoma cell growth, or caspase-7 to inhibit apoptosis in breast cancer cells. Inhibition of PAK2 activity or expression has shown promising results in research studies and may represent a new therapeutic approach in cancer therapy.

References

Grebenova D., et al., 2019 Scientific Reports 9: 17171.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinases

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Chemi-Verse™ PAK2 Kinase Assay Kit

PRODUCTS

SERVICES

RESOURCES

COMPANY