Chemi-Verse™ PAK4 Kinase Assay Kit

Catalog #

82181

$535 *

Size: 96 reactions

Qty

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ PAK4 Kinase Assay Kit is designed to measure PAK4 (p21-activated kinase 4) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant PAK4 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

KIAA1142, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4 (PAK-4), PAK4

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Product Data Gallery

Inhibition of PAK4 kinase activity by Staurosporine.

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
	PAK4*	1 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
	PAKtide, 1 mg/ml	500 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

O96013

Background

PAK4 (p-12-activated kinase 4) is a member of the PAK family of serine/threonine kinases. The PAK family is highly evolutionary conserved and regulates cell survival, apoptosis, cytoskeleton dynamics, and other critical cellular functions. PAK4 belongs to group II, which can be activated by Cdc42 (cell division control protein 42) only as opposed to group I, which depends on either Rac1 or Cdc42.I It is ubiquitously expressed, participating in cytoskeleton remodeling, cell motility, apoptosis, and metastasis. PAK4 is regulated by HGF (hepatocyte growth factor), PKA (protein kinase A), CDK5RAP3 (CDK5 regulatory subunit associated protein 3) and SH3RF2 (SH3 domain containing ring finger 2), and in turn phosphorylates proteins like p120-catenin, SMAD2 and β-catenin. Upregulation of PAK4 has been found in multiple cancers, such as ovarian, breast and gastric cancer. Inhibition of PAK4 leads to milder cancer cell migration and invasion, and to regulation of the immune response to cancer, such as T cell infiltration. Interestingly, it was observed that the cell line HCT116, a KRAS-driven cell line, is highly sensitive to PAK4 inhibition. The development of specific inhibitors targeting PAK4, to be used alone or as combination therapy, will greatly benefit the cancer therapy field.

References

Wang H., et al., 2021 Expert Opin Ther Pat. 31(11): 977-987.
Li Y., et al., Front Pharmacol. 13:956220.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Cancer Therapy Target

Regulatory Status

Research Use Only

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Chemi-Verse™ PAK4 Kinase Assay Kit

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