MALT1, FLAG-Tag (Mouse) Recombinant

Catalog #

100054

$435 *

Size: 20 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Mouse MALT1 also known as IMD12, MLT, MLT1, PCASP1, and MALT1 paracaspase, GenBank Accession No. NM_172833, a.a. 2-832 (end), and expressed in a Baculovirus infected Sf9 cell expression system. MW = 93 kDa.

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Synonyms

IMD12, MLT, MLT1, PCASP1, MALT1 paracaspase

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Western Blot

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

Sf9

Purity

>84%

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol 100ng/ul flag peptide

93 kDa

Amino Acids

2-832 (end)

Genbank #

NM_172833

Tag(s)

N-terminal FLAG-Tag

References

1. Lijun, S., et al., Molecular Cell. 2004. May 14;3: 289-301.

2. Lucas, P., et al., JBC. 2001. June 276: 19012-19019.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

The assay was performed by fluorescence using a recombinant mMALT1 and Ac-LRSR-AMC as substrate. The fluorescence at (ex 365 em 460 nm) is correlated with the amount of product formed.All of the reactions were conducted at 30 ºC. The reaction mixture is in either 96 well black nunc plates or 384 well black Greiner plates as indicated. The reaction in MALT1 Assay Buffer (50 mM MES, 150 mM NaCl, 0.1% CHAPS, 1 M Na.citrate, 10 mM DTT added fresh, pH 6.8) contains 0-500 µM Ac-LRSR-AMC, and 0- 2 µg MALT1. The reaction mixture incubated for up to 3h while reading the fluorescence signal. For the negative control (blank), assay buffer was added instead of the enzyme. Absorption signals were measured using a Tecan Infinite M1000 plate reader.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Proteases

Regulatory Status

Research Use Only