Luciferase, His-Tag (Firefly) Recombinant

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Catalog #

100576

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Description

Recombinant thermostable variant of Photuris pensylvanica (Firefly) Luciferase, also known as Luciferin 4-monooxygenase. Full length, encompassing amino acids 1-545(end). This construct contains a C-terminal His-tag (6xHis) The recombinant protein was affinity purified.

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Synonyms

Luciferin 4-monooxygenase

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Firefly

Host Species/Expression System

E. coli

Purity

≥90%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 150 mM Imidazole and 20% glycerol.

61 kDa

Amino Acids

1-545(full length)

UniProt #

Q27757

Tag(s)

C-terminal His-tag

References

1. DE Wet, J.R., et al., Molec. Cell. Bio. 1987 Feb; 7(2):725-737.
2. Brasier, AR., et al., Euro. PMC. 1989 Oct; 7(10):1116-1122.

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Datasheets/Protocols

100576 Lot 200220-B

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Luciferase, His-Tag (Firefly) Recombinant

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