LTβR:LIGHT[Biotinylated] Inhibitor Screening Assay Kit

Catalog #

79808

$1,040 *

Size: 96 Reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The LTβR:LIGHT[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of LIGHT binding to LTβR . This kit comes in a convenient 96-well format, with biotin-labeled LIGHT, purified LTβR , streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled LIGHT by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LTβR is coated on a 96-well plate. Next, biotinylated LIGHT is incubated with LTβR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against LTβR:LIGHT[Biotinylated]? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

TNFSF14, lymphotoxin beta receptor, LTBR, LTBR-Fc fusion protein, Tumor Necrosis Factor C Receptor, TNFCR, CD18

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips

Format

Catalog Number	Component	Amount	Storage
71112	Human LTBR, Fc-Fusion	10 µg	-80 °C	Avoid freeze/ thaw cycles!
100032	LIGHT, Biotin-Labeled	10 µg	-80 °C
79311	3x Immuno Buffer 1	50 ml	-20 °C
79728	Blocking Buffer 2	50 ml	+4 °C
79742	Streptavidin-HRP	15 µl	-20 °C
79742	ELISA ECL Substrate A	6 ml	RT
79742	ELISA ECL Substrate B	6 ml	RT
79699	96-well white microplate	1	+4°C

UniProt #

LTβR: P36941; LIGHT: O43557

Background

Lymphotoxin β receptor (LTβR) and herpes virus entry mediator (HVEM) are the physiological receptors of LIGHT (tumor necrosis factor superfamily member 14 (TNFSF14) or CD258). The activation of the LTβR/HVEM:LIGHT co-signaling pathways induces the production of pro-inflammatory molecules, like NF-B, chemokines and adhesion molecules. LTβR is up regulated in hepatitis and hepatocellular carcinoma, and influences lipid metabolism and atherosclerosis. It also regulates cell growth and can initiate inflammation related carcinogenesis.

References

Chang, J. H., et al. J Biomed Sci 2005, 12(2): 363-375.
Del Rio, M.L., et al. J. Leukoc. Biol. 2010, 87(2): 223-235

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Warnings

Avoid multiple freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only