LTβR:LIGHT[Biotinylated] Inhibitor Screening Assay Kit
The LTβR:LIGHT[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of LIGHT binding to LTβR . This kit comes in a convenient 96-well format, with biotin-labeled LIGHT, purified LTβR , streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled LIGHT by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LTβR is coated on a 96-well plate. Next, biotinylated LIGHT is incubated with LTβR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
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PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Catalog Number |
Component | Amount | Storage | |
71112 | Human LTBR, Fc-Fusion | 10 µg | -80 °C | Avoid freeze/ thaw cycles! |
100032 | LIGHT, Biotin-Labeled | 10 µg | -80 °C | |
79311 | 3x Immuno Buffer 1 | 50 ml | -20 °C | |
79728 | Blocking Buffer 2 | 50 ml | +4 °C | |
79742 | Streptavidin-HRP | 15 µl | -20 °C | |
79742 | ELISA ECL Substrate A | 6 ml | RT | |
ELISA ECL Substrate B | 6 ml | RT | ||
79699 | 96-well white microplate | 1 | +4°C |
Chang, J. H., et al. J Biomed Sci 2005, 12(2): 363-375.
Del Rio, M.L., et al. J. Leukoc. Biol. 2010, 87(2): 223-235