LKB1/MO25/STRAD Complex Recombinant

Catalog #

40069

$370 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Complex of human LKB1, also known as STK11, GenBank Accession No. NM_000455, a.a. 2-433, with N-terminal Histag, calculated MW=49 kDa (LKB1 runs anomalously on SDS-PAGE), MO25 GenBank Accession No. NM_016289, a.a. 2-341, with Nterminal GST-tag, calculated MW=66 kDa and STRAD, GenBank Accession No. NM_001003787, a.a. 2-431, with N-terminal GST-tag, calculated MW=74 kDa, individually expressed in Sf9 cells using a Baculovirus expression system.

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Synonyms

LKB1, STK11, PJS, Liver Kinase B1, STRAD, STE20-related kinase adaptor, LYK5, PMSE, MO25, Calcium Binding Protein 39

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, and 3 mM DTT.

189 kDa (complex)

Amino Acids

LKB1 2–433, MO25 2–341, STRAD 2–431

Specific Activity

24 pmol/min/µg

Genbank #

STK11 (NM_000455), MO25 (NM_016289), STRAD (NM_001003787)

UniProt #

LKB1: Q15831; MO25: Q9Y376; STRAD: Q7RTN6

Tag(s)

STK11, N-terminal His-tag, MO25, N-terminal GST-tag, STRAD, N-terminal GST-tag

References

1. Yoo, L.I., et al. (2002). Nat. Rev. Cancer 2(7): 529-535.
2. Katajisto P, et al. (2007). Biochim. Biophys. Acta 1775 (1): 63–75.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

100 µM ATP and 0.2 mg/ml LKBtide peptide was used as a substrate. Reaction was incubated at 30°C for 30 min. Reaction progress was monitored using ADP-Glo reagents (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40069 Lot 100524-2.pdf

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LKB1/MO25/STRAD Complex Recombinant

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