KRAS, Isoform B, His-Tag, BODIPY-GDP Loaded Recombinant

Catalog #

100886

$395 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Recombinant human KRAS (GTPase), isoform b, encompassing amino acids 2-185. This construct contains a N-terminal His-tag (6xHis). The protein was affinity purified and was loaded with BODIPY-GDP. Unbound BODIPY-GDP was removed by spin column and the protein is ready for a GDP/GTP exchange assay.

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Synonyms

C-K-RAS, CFC2, K-ras, kRas, KRAS proto-oncogene, GTPase, Kirsten Rat Sarcoma virus, NS, NS3, OES, RALD, KRAS1, KRAS2, RASK2, KI-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, c-Ki-ras, c-Ki-ras2

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

KRAS BODIPY-GDP Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

KRAS (His-2-185)-(BODIPY-GDP)

Host Species/Expression System

Sf9 insect cells

Purity

72%

Supplied As

Aqueous buffer solution.

Formulation

20 mM HEPES, pH 7.4, 150 mM NaCl and 1 mM DTT

22 kDa

Amino Acids

2-185

Genbank #

NM_004985

UniProt #

P01116

Tag(s)

N-terminal His-tag

Label

BODIPY™ FL GDP ((ThermoFisher Scientific, Guanosine 5'-Diphosphate, BODIPY™ FL 2'-(or-3')-O-(N-(2-Aminoethyl) Urethane), Bis (Triethylammonium) Salt), is a mixed isomer in which the BODIPY® FL fluorophore has been attached to the 2’ or 3’ position of the ribose ring via a linker. Invitrogen BODIPY® FL dye is a green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Alexa Fluor 488. It is characterized by a high extinction coefficient and high quantum yield and is relatively insensitive to pH changes. Its hydrophobic properties make it useful with lipophilic compounds. The dye has an excited-state lifetime of 5 nanoseconds or longer, making it useful for fluorescence polarization–based assays.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed by following the KRAS Nucleotide Exchange Assay Kit (BPS Bioscience, #79859) protocols with BODIPY-GDP-loaded KRAS(WT) concentrated at 0.12 mg/ml and GTP titrated 3-fold from 100 μM – 0 μM.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for KRAS(WT) GDP/GTP nucleotide exchange reaction.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Ras Signaling

Regulatory Status

Research Use Only