HER2 Kinase Assay Kit
Catalog #
40721
$750
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Description
HER2 (human epidermal growth factor receptor 2) encoded by ERBB2 gene has been found to be overexpressed in certain aggressive breast cancers, suggesting it as an important drug target as well as a biomarker. The HER2 Kinase Assay Kit is designed to measure HER2 kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
This product has been cited 7 times.
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Synonyms
HER2, NEU, ErbB2, TKR1, CD340, human epidermal growth factor receptor 2, HER-2
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Product Data Gallery
Product Info
Storage and Usage
Citations7
Assay Kit Format
Luminescent
Supplied As
The HER2 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant HER2 enzyme, HER2 substrate, ATP and Kinase Buffer 1 for 100 enzyme reactions.
Materials Required But Not Supplied
Kinase-Glo MAX (Promega, #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
Format
Catalog Number |
Reagent | Amount | Storage | |
40230 | HER2 | 15 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
79334 | 5x Kinase Buffer 1 | 1.5 ml | -20°C | |
79686 | ATP (500 µM) | 100 µl | -20°C | |
40217 | PTK substrate Poly(Glu: Tyr 4: 1) (10mg/ml) |
100 µl | -20°C | |
79696 | 96-well plate, white | 1 | Room Temp. |
UniProt #
P04626
References
Tai, W. et al., Journal of Controlled Release 146(3):264-275 (2010)