HSP90α, His-tag Recombinant

Catalog #

50290

$435 *

Size: 200 µg

Qty

Bulk/Custom

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Description

Recombinant human HSP90α (heat shock protein 90 alpha family class A member 1), full length encompassing amino acids 1-732 (end). The construct contains a C-terminal His-tag (6xHis). The protein was affinity purified.

This product has been cited 7 times.

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Synonyms

HSP90a, Heat Shock Protein 90a, HSP90alpha, HSP90α, EL52, HSPN, LAP2, Heat shock 86 kDa, HSP86, HSPC1, HSPCA, Hsp89, Hsp90, Lipopolysaccharide-associated protein, LPS-associated protein 2, LAP-2, HSP89A, HSP90A, HSP90N, Hsp103, HSPCAL1, HSPCAL4, HEL-S-65p

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

HSP90α (N-Terminal Domain) inhibition curve using a fluorescence polarization assay (#50293). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations7

Species

Human

Construct

HSP90α (1-732(end)-His)

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 3 mM DTT, variable imidazole

86 kDa

Amino Acids

1-732(end)

Genbank #

NM_005348

UniProt #

P07900

Tag(s)

C-terminal His-tag

Background

Hsp90 (90 kDa heat shock protein) is a molecular chaperone that aids protein folding and quality control for a large number of client proteins. Functional Hsp90 operates as dimer and has intrinsic ATPase activity. The Hsp90 dimer acts in concert with other chaperones (e.g. Hsp70) and is regulated by a number of co-chaperones/accessory proteins (e.g. Hop, cdc37). Hsp90 has been shown to interact with > 100 proteins and some notable clients include kinases (e.g. Raf-1), nuclear hormone receptors (e.g. estrogen receptors), transcription factors (e.g. p53), GPCRs (e.g. CB2 receptors) and ion channels (e.g. CFTR). In humans, the Hsp90beta isoform is constitutively expressed whereas the Hsp90alpha isoforms is expressed under stress conditions. Hsp90 plays an important role in some tumor cell types by stabilising mutated oncogenic proteins.

References

1. Wayne N. and Bolon D.N. J Biol Chem. 2007 Nov 30;282(48):35386-95.
2. Pearl L.H., et al. Biochem J. 2008 Mar 15;410(3):439-53.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was performed according to the HSP90α N-Terminal Domain Assay Kit (BPS Bioscience #50293) with HSP90α titrated from 340 ng/rxn – 0 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Heat Shock Proteins

Regulatory Status

Research Use Only