The HIPK3 Kinase Assay Kit is designed to measure HIPK3 kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
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Synonyms
Homeodomain-interacting protein kinase 3, Androgen receptor-interacting nuclear protein kinase, ANPK, Fas-interacting serine/threonine-protein kinase, FIST, Homolog of protein kinase YAK1, HIPK3, DYRK6, FIST3, PKY
The assay kit comes in a convenient 96-well format, with enough purified recombinant HIPK3 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
*The concentration of the protein is lot-specific and will be indicated on the tube.
UniProt #
Q9H422
Background
HIPK3 (Homeodomain Interacting Protein Kinase 3) is a serine/threonine protein kinase involved in the regulation of gene transcription induced by steroid hormones such as testosterone. Also known as ANPK (Androgen receptor-interacting nuclear protein kinase), it amplifies androgen receptor-mediated transcription. The kinase phosphorylates transcription factors JUN and RNUX2 and may decrease apoptosis through phosphorylation of FADD (FAS-associated death domain protein).
Storage/Stability
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Applications
Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
The final concentration of DMSO in the assay should not exceed 1%.
Warnings
Avoid freeze/thaw cycles
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
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