HER4, GST-tag Recombinant

Catalog #

40232

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human HER4 kinase, also known as Erb-B4, Proto-Oncogene-Like Protein C-ErbB-4, and Tyrosine Kinase-Type Cell Surface Receptor HER4, GenBank Accession No. NM_005235, a.a. 708-993, with an N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=59.5 kDa.

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Synonyms

HER4, ERBB4, human epidermal growth factor receptor 4, HER-4

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Supplied As

Aqueous buffer solution.

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.

59.5 kDa

Amino Acids

708–993

Specific Activity

48 pmol/min/μg.

Genbank #

NM_005235

UniProt #

Q15303

Tag(s)

N-terminal GST-tag

References

1. Strunk, K.E. et al., Cancer Res. 67 (14), 6582- 6590 (2007).
2. Law, A.J. et al., Hum. Mol. Genet. 16 (2), 129-141 (2007).

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.2 mg/ml Poly-(Glu4:Tyr)-biotin substrate and 20 mM ATP. Reaction was done at 30°C for 40 min.Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40232 Lot 140513.pdf

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