GPRC5D Lentivirus (Macaca fascicularis/Cynomolgus)

Catalog #

78780

$850 *

Size: 500 µl x 2

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The GPRC5D (G protein-coupled receptor class C group 5 member D) Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with particles Macaca fascicularis (also known as crab-eating macaque or cynomolgus monkey) GPRC5D (XP_005570250.1) driven by a CMV promoter. The lentiviruses also transduce a hygromycin selection gene (Figure 1).

Figure 1: Schematic of the lenti-vector used to generate the cynomolgus GPRC5D Lentivirus.

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Synonyms

G-protein coupled receptor family C group 5 member D

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Product Data Gallery

Expression of GPRC5D in CHO-K1 cells using cynomolgus GPRC5D lentivirus.

Product Info

Storage and Usage

Citations

Supplied As

Two vials (500 µl x 2) of lentivirus at a titer ≥10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Formulation

The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.

Background

G protein-coupled receptor class C group 5 member D (GPRC5D) is an orphan receptor of little-known function belonging to the large family of G protein-coupled receptors. The protein is highly expressed in malignant plasma cells such multiple myeloma and is now considered a therapeutic target for antibody-based treatment of multiple myeloma. For example, CAR-T cells and GPCR5DxCD3 bispecific antibodies resulted in promising outcomes at levels well tolerated by the patients.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Applications

Expression of GPRC5D in cells of interest.
Generate stable cell lines expressing cynomolgus GPRC5D (hygromycin resistant).

Shipping Temperature

-80°C

Notes

To generate a GPRC5D stable cell line, remove the growth medium 48 hours after transduction and replace it with fresh growth medium containing the appropriate amount of hygromycin (as pre-determined from a killing curve), for antibiotic selection of transduced cells. Visit: https://bpsbioscience.com/cell-line-faq for guidelines on performing a kill curve.

Biosafety
None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For further questions, email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

CAR T-Cell Therapy

Regulatory Status

Research Use Only

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GPRC5D Lentivirus (Macaca fascicularis/Cynomolgus)

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