FYN, FLAG-Tag Recombinant

Catalog #

40433

$390 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human FYN (FYN proto-oncogene, Src family tyrosine kinase), full length, encompassing amino acids 2-537. This construct contains an N-terminal Flag-tag. The recombinant protein was affinity purified.

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Synonyms

SLK, Srk-Like Kinase

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

FYN Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

FYN (FLAG-2-537(end))

Host Species/Expression System

Sf9

Purity

≥66%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

61 kDa

Amino Acids

2–537(end)

Specific Activity

>200 pmol/min/μg.

Genbank #

NM_002037

UniProt #

P06241

Tag(s)

N-terminal FLAG-tag

References

1. Ban, K. et al., Blood, 111 (5), 2409-8 (2008)
2. Hoe, HS. et al., J.Biol.Chem 283 (10), 6288-99 (2008)

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl₂, 0.1 mg/ml BSA and 1 mM DTT) containing 20 μM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 min. at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Tyrosine Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

40433 Lot 170413

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