FYN Assay Kit
The FYN Assay Kit is designed to measure FYN activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Need us to run inhibitor screens or profile your compounds against FYN? Check out our Kinase Screening Services.
Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 0.5 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
Catalog Number |
Reagent |
Amount |
Storage |
|
40433 | FYN, FLAG-Tag | 2 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
79334 | 5x Kinase assay buffer | 1.5 ml | -20°C | |
79686 | ATP (500 μM) | 100 µl | -20°C | |
40217 | Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml) | 100 µl | -20°C | |
79696 | 96-well plate, white | 1 | Room Temp |
FYN is member of the SRC tyrosine kinase oncogene family. It plays a role in various forms of cancer and is a notable molecular target in prostate cancer. Fyn interacts with both amyloid-beta (Aβ) and tau, suggesting that inhibition of FYN has potential as a therapy for Alzheimer’s disease.
1. Resh, M.D. "Fyn, a Src family tyrosine kinase." Int. J. Biochem. Cell. Biol. 30.11 (1998): 1159-1162.
2. Umemori, H., et al. "Initial events of myelination involve Fyn tyrosine kinase signaling." Nature 367.6463 (1994): 572-576.
Application Reference(s):
1. Data Sheet FYN Assay Kit Catalog #78003 96 Reactions (2020)