MMP9 Fluorogenic (Q279R) Assay Kit

Catalog #

79915

$465 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Fluorogenic MMP9 (Q279R) Assay Kit is designed to measure MMP9 (Q279R) activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.

Need us to run inhibitor screens or profile your compounds against MMP9 (Q279R)? Check out our Protease Screening Services.

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Synonyms

MMP-9

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Fluorogenic

Supplied As

The kit comes in a convenient 96-well format, with purified MMP9 (Q279R) enzyme, fluorogenic substrate, and MMP9 assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Fluorescent microplate reader capable of reading exc/em=328 nm/393 nm

Format

Catalog Number	Component	Amount	Storage
80215	Recombinant Human MMP9 (Q279R)*	>1 µg	-80°C	Avoid freeze/ thaw cycles!
79916	1 mM MMP Substrate	50 µl	-80°C
79917	1X MMP Assay Buffer	25 ml	-20°C
79685	Black, low binding black microtiter plate	1	Room Temp

*We have given excess for ease of retrieval.

UniProt #

P14780

Background

MMP9 (matrix metalloproteinase 9), also known as Gelatinase B, is a member of the matrix metalloproteinase (MMP) family involved in the degradation of the extracellular matrix. Abnormal expression of MMP9 is observed in many diseases, including cancer and coronary artery disease. The (Q279R) mutation, found within the fibronectin type II domain, is associated with increased risk for in-transit metastasis.

References

1. Wang C., et al., 2020 Feb 12. J. Cell Physiol. doi: 10.1002/jcp.29485.
2. Owyong M., et al., 2019 Dec; Life Sci Alliance 2(6): e201800226.
3. Qu J., et al., 2019; Am. J. Cancer Res. 9(7): 1415–1428

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See data sheet for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Protease

Regulatory Status

Research Use Only