FGR Kinase Assay Kit

Catalog #

82292

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The FGR Kinase Assay Kit is designed to measure FGR kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant FGR, substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Need us to run inhibitor screens or profile your compounds against FGR? Check out our Kinase Screening and Profiling Service.

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Synonyms

Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog, Proto-oncogene c-Fgr, p55-Fgr, p58-Fgr, p58c-Fgr, Tyrosine-protein kinase Fgr

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Product Data Gallery

Inhibition of FGR kinase activity by TL02-59

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
	FGR*	5 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
40217	PTK Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml)	50 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

P09769

Background

FGR is a tissue-specific member of the Src tyrosine kinase family of proteins, and it is involved in Fcγ receptor signaling pathways in phagocytosis and mitochondrial oxidative phosphorylation. This protein is overexpressed in several cancer types, such as AML (acute myeloid leukemia), and has also been linked to Alzheimer’s disease (AD), Parkinson’s disease (PD), pain, diabetes, and schizophrenia. Activation of the FGR/SIRT1 (sirtuin 1)/PGC-1α (peroxisome proliferator-activator receptor γ coactivator 1α) pathway may also be involved in sepsis. Interestingly, it has been found that obesity can lead to FGR activation in macrophages, and contribute to inflammation, making the development of strategies targeting the mitochondrial ROS (reactive oxidative species)- FGR axis clinically relevant in obesity. A deeper understanding of the role of this protein in health and disease will open new therapeutic avenues for several FGR linked disorders.

References

Liu Y., et al., 2023 J Transl Med 21:486.
Acin-Perez R., et al., 2020 Nat Metab 2(9): 974-988.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Diabetes and Obesity

Regulatory Status

Research Use Only

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FGR Kinase Assay Kit

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