eGFP/Firefly Luciferase THP-1 Cell Line

Catalog #

82148

$1,900 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

eGFP/Firefly Luciferase THP-1 Cell Line is a THP-1 cell line expressing firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter. The cells were generated by transduction with Firefly Luciferase-eGFP lentivirus (BPS Bioscience #78741), which is a SIN (self-inactivating) lentivirus.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Luciferase activity in eGFP/Firefly Luciferase THP-1 cells and THP-1 parental cells was measured using One Step™ Luciferase Assay System (BPS Bioscience #60690).

Bright field (left) and fluorescent (right) image of GFP/Firefly Luciferase THP-1 cells.

25,000 eGFP/Firefly Luciferase THP-1 cells and parental THP-1 cells were analyzed by flow cytometry for eGFP expression.

Product Info

Storage and Usage

Citations

Host Cell Line

THP-1 cells, human monocytic, leukemia, suspension

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2M	BPS Bioscience #78181

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

THP-1 is a human monocytic leukemia cell line, derived from the peripheral blood of a 1-year-old male with acute monocytic leukemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. THP-1 cell line provides a valuable model for studying the mechanisms involved in macrophage differentiation, and for exploring the regulation of macrophage-specific genes as they relate to physiological functions displayed by these cells.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 2 (BPS Bioscience #60184): RPMI-1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2M (BPS Bioscience #78181): RPMI-1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 µg/ml of Puromycin.

Applications

In vitro and in vivo bioluminescence imaging (BLI) and fluorescence imaging.
Use as target cells in CAR-T or NK co-culture killing assays.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only