CHK1 Kinase Assay Kit
Catalog #
78418
$535
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Description
The CHK1 Kinase Assay Kit is designed to measure CHK1 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
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Product Info
Storage and Usage
Citations
Assay Kit Format
Luminescent
Supplied As
The assay kit comes in a convenient 96-well format, with enough purified recombinant CHK1 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied
- ADP-Glo® Kinase Assay (Promega #V6930)
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
Format
UniProt #
O14757
Background
CHK1 is an evolutionarily conserved serine/threonine protein kinase that operates as a cell cycle checkpoint at the G2/M phase transition in response to DNA damage. Activated CHK1 triggers a pleiotropic cellular response via phosphorylation of downstream effectors. For example, Chk1 activates and phosphorylates Wee1 kinase and regulates Cdc25C in response to DNA damage in G2 phase. Its activity is highly controlled at the G2/M phase transition.