Chemi-Verse™ TTBK2 Kinase Assay Kit

Catalog #

82225

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ TTBK2 Kinase Assay Kit is designed to measure TTBK2 (tau tubulin kinase 2) serine-threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant TTBK2 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

Tau-tubulin kinase 2, TTBK2, KIAA0847

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Product Data Gallery

Inhibition of TTBK2 kinase activity by TTBK-IN-1.

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
	TTBK2*	1.25 µg	-80°C
79334	5x Kinase Assay Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
78514	Myelin basic protein (MBP), 5 mg/ml	100 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

Q6IQ55

Background

TTBK2, or tau tubulin kinase 2, belongs to the serine-threonine kinase family, involved in primary cilium assembly. Mutations in TTBK2 are responsible for spinocerebellar ataxia 11 (SCA11), which is characterized by loss of Purkinje cells and connection to other neurons in the cerebellum. Loss of TTBK2 in a conditional knockout identified the critical role of this protein in Purkinje cells, where lack of cilia resulted in abnormal intracellular calcium levels and in loss of VGLUT2 positive synapses from dendrites. Studies into the mechanism of action of TTBK2 have identified that mutant protein can interfere with peroxisome numbers and localization, impairing the traffic of SMO (smoothened) to the cilia. Further studies are required to identify the exact functions of this protein and how best to target it when impaired.

References

Bowie E. and Goetz S., 2020 eLife 9: e51166.
Munoz-Estrada J, et al., 2023 bioRxiv: 2023.01.31.526333.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Neuroscience

Regulatory Status

Research Use Only

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Chemi-Verse™ TTBK2 Kinase Assay Kit

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