Chemi-Verse™ CDK5/p35 Kinase Assay Kit

Catalog #

82249

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ CDK5/p35 Kinase Assay Kit is designed to measure CDK5 (cyclin dependent kinase 5)/p35 kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CDK5/p35 complex, substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

CDK-5

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Product Data Gallery

Inhibition of CDK5/p35 kinase activity by Dinaciclib

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
	CDK5/p35, GST-Tag*	2 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
	Histone H1 (1 mg/ml)	500 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

Background

CDK5 (cyclin dependent kinase 5) is a serine/threonine protein kinase of the CDK family of proteins. It is expressed mainly in the brain, where it is linked to neuronal development and differentiation. CDK5 is an unconventional CDK protein, as its partner are the non-cyclin proteins p35 (early 35 kDa protein) and p39. P35 and p39 are myristoylated and recruit CDK5 to the cell membrane. Recent findings identified new roles for CDK5 in the nervous system, such as participating in neurite outgrowth, synaptic plasticity and homeostasis and circadian rhythm. Accumulation of p25, a fragment of p35, in neurons creates dysregulation of CDK5 function, and can result in Alzheimer’s disease (AD), Parkinson’s disease (PD), ALS (amyothropic lateral sclerosis) and Huntington’s disease (HD). CDK5 also participates in T cell activation, and overexpression of this protein has been found in cancer samples. It acts on increasing brain tumor stem cell numbers and can lead to upregulation of PD-L1 (programmed death ligand-1) and increase cancer cell immune evasion. Inhibition of CDK5 via small molecules and peptides is a promising area of research that can benefit cancer with neurodegenerative disorders and cancer.

References

Pao P.0C. and Tsai L.-H., 2021 Journal of Biomedical Medicine 28:79

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Neuroscience, Serine-Threonine Kinases

Regulatory Status

Research Use Only

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Chemi-Verse™ CDK5/p35 Kinase Assay Kit

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