Chemi-Verse™ AKT1 Kinase Assay Kit

Catalog #

82218

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ AKT1 Kinase Assay Kit is designed to measure AKT1 (protein kinase B) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant AKT1 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

AKT1, PKBα, PKBalpha, RACalpha, AKT

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Product Data Gallery

Inhibition of AKT1 kinase activity by A-674563

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
40003	AKT1, Active*	2.5 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
	Crosstide (5 mg/ml)	50 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

P31749

Background

AKT1, also known as Protein Kinase B Alpha (PKBα), is a serine/threonine kinase found in various cell types throughout the body, where it plays a critical role in regulating cell survival, insulin signaling, angiogenesis and tumor formation as well as controlling apoptosis. The signaling pathways involving AKT1 kinase are essential for the normal development and function of the nervous system. AKT1 has a broad number of targets and can thus regulate multiple pathways. Dysregulation of AKT1 contributes to a number of serious diseases, including cancer, diabetes, cardiovascular and neurological diseases. In cancer AKT1 is involved in tumor progression and drug resistance. For instance, the activating mutation E17K seems to be present in 7% of the estrogen-receptor positive metastatic breast cancers. The use of AKT inhibitors, such as capivasertib, alone or in combination, is currently under late-stage clinical trials and has been showing great promise for the treatment of cancer patients. A deep understanding of the mechanisms of action of AKT1 and how inhibition of AKT1 can lead to other drug resistance can be crucial to the development of new cancer therapies.

References

Smyth L., et al., 2020 Clin Cancer Res 26(15):3947-3957.
Hua H., et al., 2021 J Hematol Oncol 14:128.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinases

Regulatory Status

Research Use Only

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Chemi-Verse™ AKT1 Kinase Assay Kit

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