Chemi-Verse™ ABL2 Kinase Assay Kit

Catalog #

82220

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ ABL2 Kinase Assay Kit is designed to measure ABL2 (Abelson-Related gene protein) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant ABL2 kinase (amino acids 38-end), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

Tyrosine-protein kinase ABL2, Abelson murine leukemia viral oncogene homolog 2, Abelson tyrosine-protein kinase 2, Abelson-related gene protein, Tyrosine-protein kinase ARG, ABLL, ARG, ABL-2

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Product Data Gallery

Inhibition of ABL2 kinase activity by Staurosporine.

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
40417	ABL2, His-Tag*	1 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
	ABLtide (1 mg/ml)	500 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

P42684

Background

ABL2, also known as Abelson-related gene protein or ABL proto-oncogene 2, is a cytoplasmic tyrosine kinase of the Abelson tyrosine kinase family. ABLs are involved in development and cellular homeostasis and become abnormally activated in the presence of insults, such as inflammation, tissue injury, cancer, neural degeneration and other diseases. They respond to growth factors, chemokines, oxidative stress, and DNA damage by promoting cytoskeleton rearrangements. For instance, in solid tumors their activation can lead to invadopodia formation and EMT (epithelial-mesenchymal transition), which promotes metastasis and resistance to chemotherapy. Inhibition of ALB2 by allosteric inhibitors and PROTACs may prove beneficial in creating sensitivity of the patients to standard methods of cancer treatment. A deeper understanding of the mode of action of ABL2 and results of its inhibition will move the cancer therapy field forward.

References

Luttman J., et al., 2021 Cell Communications and Signaling 19:59.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinases

Regulatory Status

Research Use Only

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Chemi-Verse™ ABL2 Kinase Assay Kit

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