CD137:CD137L[Biotinylated] Inhibitor Screening Assay Kit

Catalog #

78590

$1,130 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The CD137:CD137L [Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD137:CD137L interaction. The assay takes advantage of the highly sensitive detection of biotin-labeled CD137L by streptavidin-HRP. First, CD137 is coated on a 96-well plate. Next, CD137L-biotin is incubated with CD137. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against CD137:CD137L[Biotinylated]? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

4-1BB, Tumor Necrosis Factor Receptor Superfamily member 9, TNFRSF9, ILA, 4-1BB ligand, 4-1BBL, tumor necrosis factor ligand superfamily member 9, TNFSF9

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Product Data Gallery

CD137:CD137L[Biotinylated] Interaction Binding and Inhibition

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Species

Human

Supplied As

The CD137:CD137L[Biotinylated] Inhibitor Screening Assay Kit comes in a convenient 96-well format, with biotin-labeled CD137L, purified CD137, streptavidin-labeled HRP, and assay buffer for 100 reactions.

Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Rotating or rocker platform

Format

Catalog #	Name	Amount	Storage
71170	CD137 (4-1BB), Fc fusion*	10 µg	-80°C
100238	CD137L (4-1BB ligand), His, Avi, Biotin	2 µg	-80°C
79742	Streptavidin-HRP	10 µl	4°C
79311	3x Immuno Buffer 1	50 ml	-20°C
79728	Blocking Buffer 2	50 ml	4°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	Room Temp
79670	ELISA ECL substrate B (brown bottle)	6 ml	Room Temp
79696	96-well plate, white	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube

UniProt #

CD137: Q07011; CD137L: P41273

Background

CD137, also known as 4-1BB, is a prototype co-stimulatory molecule expressed on T cells. CD137 interaction with its cognate ligand, CD137L, also known as 4-1BBL, results in increased T cell activation and proliferation. Accumulating evidence shows a role for CD137:CD137L signaling in inflammation, suggesting that inhibition of this pathway may provide a therapeutic avenue to treat autoimmune and inflammatory diseases.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Screen and profile inhibitors of CD137 binding to CD137L.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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CD137:CD137L[Biotinylated] Inhibitor Screening Assay Kit

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