CD137:CD137L[Biotinylated] Inhibitor Screening Assay Kit
The CD137:CD137L [Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD137:CD137L interaction. The assay takes advantage of the highly sensitive detection of biotin-labeled CD137L by streptavidin-HRP. First, CD137 is coated on a 96-well plate. Next, CD137L-biotin is incubated with CD137. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
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- PBS (Phosphate buffered saline)
- Luminometer or microplate reader capable of reading chemiluminescence
- Rotating or rocker platform
Catalog # | Name | Amount | Storage |
71170 | CD137 (4-1BB), Fc fusion* | 10 µg | -80°C |
100239 | CD137L (4-1BB ligand), His, Avi, Biotin | 2 µg | -80°C |
79742 | Streptavidin-HRP | 10 µl | 4°C |
79311 | 3x Immuno Buffer 1 | 50 ml | -20°C |
79728 | Blocking Buffer 2 | 50 ml | 4°C |
79670 | ELISA ECL substrate A (transparent bottle) | 6 ml | Room Temp |
ELISA ECL substrate B (brown bottle) | 6 ml | Room Temp | |
79696 | 96-well plate, white | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube
CD137, also known as 4-1BB, is a prototype co-stimulatory molecule expressed on T cells. CD137 interaction with its cognate ligand, CD137L, also known as 4-1BBL, results in increased T cell activation and proliferation. Accumulating evidence shows a role for CD137:CD137L signaling in inflammation, suggesting that inhibition of this pathway may provide a therapeutic avenue to treat autoimmune and inflammatory diseases.