CD137 (4-1BB), Fc Fusion, Avi-tag Biotin-Labeled HiP™ Recombinant

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Catalog #

71171

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Description

Recombinant human CD137 (secreted), encompassing amino acids 24-186. The protein is fused at the C-terminus with the Fc fragment of human IgG1 followed by an Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.

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Synonyms

Tumor Necrosis Factor Receptor Superfamily member 9; TNFRSF9; ILA; 4-1BB; CDw137

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

CD137[Biotinylated]:CD137L Interaction

Biotin-Avidin Pulldown

Gel Filtration

CD137[Biotinylated]:CD137L inhibition curve using a chemiluminescent assay (#72025). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

CD137 (4-1BB) (24-186-Fc(IgG1)-Avi)-(biotin)

Host Species/Expression System

HEK293

Purity

≥90%

Supplied As

Aqueous buffer solution.

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

46 kDa + glycans

Amino Acids

24-186

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Genbank #

NM_001561

UniProt #

Q07011

Tag(s)

C-terminal Fc fusion, Avi-Tag

Label

This protein is enzymatically biotin-labeled using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%.

Storage/Stability

At least 6 months at –80°C

Assay Conditions

Assay was performed according to the CD137[Biotinylated]:CD137L Inhibitor Screening Assay Kit (BPS Bioscience, #72025) with CD137 [Biotinylated] enzyme titrated from 80 ng/rxn – 0.08 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

71171 Lot 210415

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CD137 (4-1BB), Fc Fusion, Avi-tag Biotin-Labeled HiP™ Recombinant

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