CD112R (PVRIG), Fc-Fusion, Avi-Tag (Human) HiP™ Recombinant

Catalog #

79116

$320 *

Size: 100 μg

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Description

Recombinant human CD112R, also known as PVRIG (Poliovirus receptor-related Immunoglobulin domain-containing protein), encompassing amino acids 41-172. This construct contains a C-terminal Human IgG1, Fc region followed by an Avi-tag™. This recombinant protein was affinity purified.

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Synonyms

Poliovirus Receptor-related Immunoglobulin Domain-containing Protein, Transmembrane Protein PVRIG

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

CD112R:CD112 [Biotinylated] Binding Activity

PVRIG:PVRL2-Biotin Neutralizing Assay

Gel Filtration Curve

Product Info

Storage and Usage

Citations

Species

Human

Construct

CD112R (41-172-Fc(IgG1)-Avi)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

42 kDa + glycans

Amino Acids

41-172

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Aggregation

<10%

Genbank #

NM_024070

UniProt #

Q6DKI7

Tag(s)

C-terminal Fc-Fusion- Avi-Tag

Background

PVRIG, also known as Poliovirus receptor related immunoglobulin domain containing protein or CD112R, is a natural killer (NK) cell immune checkpoint receptor, and it is also found in CD8+ T cells. PVRIG works as an inhibitory receptor and recognizes CD112 (also known as poliovirus receptor-related 2, PVRL2 or nectin-2) as its ligand. This binding results in lower responses of NK and T cells towards cancer cells. In T cells it inhibits the nuclear factor of activated T cells (NFAT). Blocking the interaction between PVRIG and its ligand, for instance with an antibody directed towards PVRIG, represents a novel strategy in checkpoint blockade immunotherapy.

References

1. Bottino, C., et al., J. Exp. Med. 2003; 198(4): 557-567.

2. Pende, D., et al., Blood. 2005; 105(5): 2066-2073

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay run according to CD112R:CD112 [Biotinylated] Inhibitor Screening Assay Kit (BPS Bioscience #79732). 100 ng of CD112R was used to coat plates overnight at 4°C. Various amounts of CD112 [Biotinylated] were incubated for 2 hours at room temperature.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Notes

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

79116 Lot 220621

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CD112R (PVRIG), Fc-Fusion, Avi-Tag (Human) HiP™ Recombinant

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