c-CBL, GST-Tag Recombinant

Catalog #

100370

$345 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human c-CBL, also known as CBL-2, Cbl Proto-Oncogene, Signal Transduction Protein CBL, and Cbl Proto-Oncogene, E3 Ubiquitin Protein Ligase, Genbank Accession No. NM_005188, a.a. 49-434 with N-terminal GST-tag, expressed in an E. coli expression system. MW = 71 kDa.

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Synonyms

CBL-2, Cbl Proto-Oncogene, Signal Transduction Protein CBL; Cbl Proto-Oncogene, E3 Ubiquitin Protein Ligase, c-CBL

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

c-CBL inhibition curve using a TR-FRET assay (#79786). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.04% Tween20, 20% glycerol, 16 mM glutathione, 3 mM DTT.

71 kDa

Amino Acids

49-434

Genbank #

NM_005188

UniProt #

P22681

Tag(s)

N-terminal GST-Tag

References

Griffiths, E.K., Molec. and Cellular Bio. 2003; Oct. 23(21):7708-7718

Keane, M.M., Oncogene, 1999 June; 18: 3365-3375.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

10 µL of reaction mixture contains 50 mM Tris, pH 7.4, 5 mM MgCl2, 0.01% Tween, 0.2 μM Ub[Biotin], 50 nM Ube1, 1 mM ATP, 2 μM of UBCH5b, and various amount of CBL-B and C-CBL. The reaction was initiated with the addition of ATP, and incubated at 30°C for 3 hours. Tb labeled anti-GST antibody donor and xl665 labeled acceptor was added and incubated at room temperature for one hour.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Ubiquitin

Regulatory Status

Research Use Only