Anti–HDAC3 monoclonal antibody

Catalog #

25289

$415 *

Size: 50 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Monoclonal antibody raised in mouse against human HDAC3 (Histone deacetylase 3), using a KLH–conjugated synthetic peptide containing a sequence from the C–terminal region of the protein.

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Synonyms

HD3, RPD3, RPD3–2, SMAP45, HDAC-3

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Product Data Gallery

Product Info

Storage and Usage

Citations

Isotype

IgG1

Clonality

monoclonal

Host Species/Expression System

Mouse

Purification

Protein A purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide and 0.05% ProClin 300

Concentration

50 µg/ 25 µl

Immunogen

synthetic peptide

Background

HDAC3 (UniProt/Swiss–Prot entry O15379) catalyses the deacetylation of lysine residues in the N–terminal part of the core histones (H2A, H2B, H3 and H4). Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression and HDAC activity is associated with gene repression. Histone deacetylation is established by the formation of large multiprotein complexes. HDAC3 may bind to the zinc–finger transcription factor YY1, thereby regulating transcription. It is also able to modulate cell growth and apoptosis through the interaction with p53 and is thought to be essential for the repression of the POU1F1 transcription factor.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

ChIP results obtained with the monoclonal antibody directed against HDAC3
ChIP assays were performed using human HeLa cells, the monoclonal antibody against HDAC3 (Cat. # 25289) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 10,000 cells. A titration of the antibody consisting of 1, 2, 5, and 10 µg per ChIP experiment was analyzed. IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH, and for the coding region of p21, a known target gene of HDAC3. Figure 4 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Immunofluorescence using the monoclonal antibody directed against HDAC3
HeLa cells were stained with the antibody against HDAC3 (Cat. # 25289) and with DAPI. Cells were fixed with 4% formaldehyde for 10 min. and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Applications

ChIP (2 µg/ChIP)
IF (1:500)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody amount per IP should be determined by the end–user. We recommend testing 1–5 µg per IP

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–HDAC3 monoclonal antibody

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